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in vitro azadirachtin production by hairy root cultivation

in vitro azadirachtin production by hairy root cultivation

In vitro azadirachtin production by hairy root cultivation ...

Azadirachtin, a well-known biopesticide is a secondary metabolite conventionally extracted from the seeds of Azadirachta indica. The present study involved in vitro azadirachtin production by developing hairy roots of A. indica via Agrobacterium rhizogenes-mediated transformation of A. indica explants.

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In Vitro Azadirachtin Production by Hairy Root Cultivation ...

10%  Nov 15, 2011  Hence, the growth cycle period for the hairy root culture could be reduced from 40 to 25 days in order to achieve maximum azadirachtin production from the in vitro cultivation of A. indica liquid hairy root culture. Scale-up of A. indica Hairy Root Cultivation in Liquid-Phase and Gas-Phase Reactors Stirred Tank Reactor

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In Vitro Azadirachtin Production by Hairy Root Cultivation ...

In Vitro Azadirachtin Production by Hairy Root Cultivation of Azadirachta indica in Nutrient Mist Bioreactor November 2011 Applied Biochemistry and Biotechnology 166(2):365-78

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Azadirachtin production by hairy root cultivation of ...

Further, batch cultivation of hairy roots was carried out in a novel liquid-phase bioreactor configuration (modified stirred tank reactor with polyurethane foam as root support) to investigate the possible scale-up of the established A. indica hairy root culture. A biomass production of 15.2 g/l with azadirachtin accumulation in the hairy roots ...

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Azadirachtin production by hairy root cultivation of ...

May 13, 2012  In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Srivastava S, Srivastava AK. Appl Biochem Biotechnol, 166(2):365-378, 15 Nov 2011 Cited by: 8 articles PMID: 22083394

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Effect of elicitors and precursors on azadirachtin ...

The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica. Improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogenous addition of precursors and elicitors in the ...

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Azadirachtin production by hairy root cultivation of ...

May 13, 2012  In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Srivastava S, Srivastava AK. Appl Biochem Biotechnol, 166(2):365-378, 15 Nov 2011 Cited by: 8 articles PMID: 22083394

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Azadirachtin production by hairy root cultivation of ...

May 13, 2012  Present investigation involves hairy root cultivation of Azadirachta indica in a modified stirred tank reactor under optimized culture conditions for maximum volumetric productivity of azadirachtin. The selected hairy root line (Az-35) was induced via Agrobacterium rhizogenes LBA 920-mediated transformation of A. indica leaf explants (Coimbatore variety, India).

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Azadirachtin production by hairy root cultivation of ...

May 13, 2012  This included cultivation azadirachtin production was highest on 15th day of the growth cycle (2.96 mg/(l day)) as a result combined addition of a fast-growing, high-yielding hairy root line of A. indica under optimum cultivation conditions in the selected bio- of the elicitor and precursor on 15th day could possibly enhance the azadirachtin ...

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Azadirachtin production by hairy root cultivation of ...

A biomass production of 15.2 g/l with azadirachtin accumulation in the hairy roots of 6.4 mg/g (97.28 mg/l) could be achieved after 25 days of the batch cultivation period, which was ~27 and ~14 % ...

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Production of biopesticide Azadirachtin by Hairy Root of A ...

View Production of biopesticide Azadirachtin by Hairy Root of Adica in Liquid phase bioreactors.pdf from BIOTECH 15BT201 at SRM University. Appl Biochem Biotechnol (2013) 171:1351–1361 DOI

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Production of biopesticide azadirachtin using plant cell ...

Apr 17, 2017  Production of biopesticide azadirachtin using plant cell and hairy root cultures ... the inconsistent content and purity of the metabolites in whole plant makes it imperative to tap the potential of in vitro plant tissue ... This review gives a summarized literature of the applied research and achievements in plant cell/hairy cultures of A ...

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List of Publications – Department of Biotechnology

In vitro cultivation of azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Applied Biochemistry and Biotechnology, 166, 365-378, 2012. Applied Biochemistry and Biotechnology, 166, 365-378, 2012.

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Mass production of Ajmalicine by bioreactor cultivation of ...

Mar 15, 2017  In vitro azadirachtin production by hairy root cultivation of azadirachta indica in nutrient mist bioreactor Appl. Biochem. Biotechnol. , 166 ( 2012 ) , pp. 365 - 378

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Plant Cell-Batch Cultivation (Theory) : Bioreactor ...

Hence, in vitro cultivation of plant cells in bioreactor is considered to be an attractive alternative and viable approach for the production of these compounds. Bio-pesticide production from A. indica . Azadirachtin is presently being isolated by solvent extraction from the seed kernels of A. indica.

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Hairy Root Culture: Bioreactor Design and Process ...

Apr 19, 2013  Srivastava S, Srivastava AK (2012b) In vitro Azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Appl

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‪Smita Srivastava‬ - ‪Google Scholar‬

Azadirachtin production by hairy root cultivation of Azadirachta indica in a modified stirred tank reactor. S Srivastava, AK Srivastava. Bioprocess and biosystems engineering 35 (9), 1549-1553. , 2012. 23. 2012. Cyclotides from the Indian Medicinal Plant Viola

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reinforced concrete pipe Companies and Suppliers. Forterra Pressure Pipe is the leading manufacturer and a principal supplier of highly engineered concrete and steel pipe systems in the United States and Canada, with more than 80 million feet of pipe in service today.

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Thèses : « Sementes - Extratos » – Grafiati

This work aims to study the effect of four extracts of Neem seed oil (Azadirachta indica) containing 2.000, 5.000, 9.000 and 10.000 ppm of azadirachtin (AZA) quantified by high performance liquid chromatography (HPLC) on engorged females and larvae of R. (B.) microplus, in vitro.

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Production of biopesticide azadirachtin using plant cell ...

Apr 17, 2017  Production of biopesticide azadirachtin using plant cell and hairy root cultures ... the inconsistent content and purity of the metabolites in whole plant makes it imperative to tap the potential of in vitro plant tissue ... This review gives a summarized literature of the applied research and achievements in plant cell/hairy

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Statistical medium optimization for enhanced azadirachtin ...

production of 13.3 gl-1 dry weight. To enhance the production of azadirachtin, a Plackett-Burman experimental design protocol was used to identify key medium nutrients and concentrations to support high root biomass production and azadirachtin accumulation in hairy roots. The optimal nutrients and concentrations were as follows: 40 gF1

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*PUBLICATIONS*

Smita Srivastava, "In vitro azadirachtin (biopesticide) production by hairy root cultivation of Azadirachta indica in a modified bubble column reactor", Invited paper presentation under ISCA Young Scientists Award Programme for 2011-12 in Engineering Sciences, October 14, 2011, KIIT University, Bhubaneswar, India.

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Hairy Root Culture: Bioreactor Design and Process ...

Apr 19, 2013  Srivastava S, Srivastava AK (2012b) In vitro Azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Appl

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Induction of hairy root cultures of Azadirachta indica A ...

T1 - Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites. AU - Allan, Eunice Jessie. AU - Eeswara, J. P. AU - Jarvis, A. P. AU - Mordue, Anne Jennifer. AU - Morgan, E. D. AU - Stuchbury, Trevor. PY - 2002. Y1 -

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Bioprocessing of differentiated plant in vitro systems ...

Oct 16, 2012  Azadirachtin: Hairy roots: 27: Beta vulgaris L. Betalains, phenolics, peroxidase ... Many types of reactor configurations for hairy root cultivation have been used, ... the evaluation of a bioreactor design should be a step of the integrated optimization process of bioactive substance production by differentiated plant in vitro systems.

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First Report of Agrobacterium rhizogenes-induced Hairy ...

The hairy root culture (HRC)-based in vitro productions were reported for bioactive molecules such as shikonins, azadirachtin, camptothecins, harpagide, ginsenosides, paclitaxel, hyoscyamine and scopolamine [1 1 Satdive RK, Fulzele DP, Eapen S. Enhanced production of azadirachtin by hairy root cultures of Azadirachta indica A. Juss by ...

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PLANT CELL CULTIVATION OF A INDICA IN BIOREACTOR FOR

A pre-optimized computer simulated continuous cultivation strategy with cell retention was experimentally implemented which featured high biomass and azadirachtin production from A indica cells in the bioreactor. The experimental verification resulted in a biomass accumulation of 61.4 g/L and azadirachtin production of 751.9 mg/L with 14% ...

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(PDF) Bioprocessing of differentiated plant in vitro ...

Bioprocessing of differentiated plant in vitro systems. Download. ... in plant cell culture for producing useful products. By Melissa Towler. Plant Cell Cultures: Bioreactors for Industrial Production. ... MINI-REVIEW Hairy root type plant in vitro systems as sources of bioactive substances. By Con Kjm Kjm. Hairy root type plant in vitro ...

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Hairy Root Culture - an overview ScienceDirect Topics

A. tumefaciens and A. rhizogenes infected root furnish a promising platform for the genetic manipulation of plant cells. As discussed A. rhizogenes-mediated transformation of plants can be used in a similar manner to that used during A. tumefaciens transformation. Hairy root syndrome and production of transgenic hairy root cultures by genetic manipulation can be achieved by A. rhizogenes ...

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azadirachtin, 11141-17-6

PubMed:In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. PubMed:In vitro and in vivo antiparasitic activity of Azadirachtin against Argulus spp. in Carassius auratus (Linn. 1758).

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Enhanced production of artemisinin by hairy root ...

It is primarily produced by a plant Artemisia annua, however, its supply from plant is significantly lower than its huge demand and therefore alternative in vitro production routes are sought. Hairy root cultivation could be one such alternative production protocol. Agrobacterium rhizogenes was used to induce hairy roots of A. annua.

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Vol. 48, No. 1, January/February 2012 of In Vitro Cellular ...

Description: In Vitro Cellular and Developmental Biology – Plant publishes timely, peer-reviewed papers for the growing body of researchers involved in cellular, molecular or developmental biology using in vitro grown or maintained organs, tissues, or cells derived from plants. The journal provides original research devoted to the advancement and dissemination of basic and applied knowledge ...

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Plant Cell Culture Case study Alison

And if I find the need then I will also talk aboutthe hairy root culture process. So, for the plant cell suspension culturesthe objectives which were laid down were to establish the cell suspension of A indicafor production of azadirachtin under in vitro condition.

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